Selected Research Reports
Wolfe MS, Citron M, Diehl TS, Xia W, Donkor IO, Selkoe DJ. “
A substrate-based difluoroketone selectively inhibits Alzheimer’s g-Secretase Activity.”
J. Biol. Chem., 1998; 41:6-9.
Wolfe MS, Xia W, Moore CL,
Leatherwood DD, Ostaszewski B, Rahmati T, Donkor IO,
and Selkoe DJ. “Peptidomimetic
Probes and molecular modeling suggest that Alzheimer’s
gamma-secretase is an intramembrane-cleaving aspartyl protease.” Biochemistry
1999, 38, 4720-7.
Wolfe MS, Xia W, Ostaszewski BL,
Diehl TS, Kimberley TS, and Selkoe DJ. “Two
Transmembrane aspartates in presenilin-
1 required for presenilin endoproteolysis and gamma-secretase
activity.” Nature
1999, 398, 513-7.
Esler WP, Kimberly WT,
Ostaszewski BL, Diehl TS, Moore CL, Tsai J-Y, Rahmati T,
Xia W, Selkoe DJ,
and Wolfe MS. “Transition-State
Analogue inhibitors of gamma-secretase bind directly
to presenilin-1.” Nature Cell Biology 2000,2(7),
428-34.
Esler WP, Kimberly WT,
Ostaszewski BL, Ye W, Diehl TS, Selkoe DJ, and
Wolfe MS. “Activity-dependent
isolation of the presenilin/gamma-secretase complex
reveals nicastrin and a gamma substrate.” Proc. Natl. Acad. Sci. USA 2002,
99, 2720-5.
Micchelli CA, Esler WP,
Kimberly WT, Jack C, Berezovska O, Kornilova A, Hyman BT,
Perrimon N, and Wolfe MS. “gamma-secretase/presenilin
inhibitors for Alzheimer’s disease phenocopy Notch
mutations in Drosophila.” FASEB J, 2003, 17,
79-81.
Kimberly WT, Esler WP, Ye W,
Ostaszewski BL, Gao J, Diehl TS, Selkoe DJ, and
Wolfe MS. “Notch and
amyloid precursor protein are cleaved by similar
gamma-secretase(s).” Biochemistry,
2003, 42, 137-44.
Kornilova AY, Das C, and Wolfe MS. "Differential
effects of inhibitors on the gamma-secretase complex: mechanistic
implications.” J. Biol. Chem., 2003, 278, 16470-3.
Kimberly WT, Ostaszewski BL, Ye W,
LaVoie MJ, Wolfe MS and Selkoe DJ. "Gamma-Secretase
is a membrane protein complex of presenilin, nicastrin,
aph-1 and
pen-2. Proc.
Natl. Acad. Sci. USA, 2003, 100, 6382-6387.
Das C, Berezovska O, Diehl TS, Genet C, Buldyrev I, Tsai JY, Hyman BT, Wolfe MS. “Designed helical peptides inhibit an intramembrane protease.”Biochemistry,
2003, 42, 137-44.
Fraering PC, LaVoie MJ, Ye W, Ostaszewski BL, Kimberly WT, Selkoe DJ, Wolfe MS. “Detergent-dependent dissociation of active g-secretase reveals an interaction between Pen-2 and PS1-NTF and offers a model for subunit organization within the complex.”
Biochemistry,
2004; 43: 323-33.
Fraering PC, Ye W, Strub JM, Dolios G, LaVoie MJ, Ostaszewski BL, van Dorsselaer A, Wang R, Selkoe DJ, Wolfe MS. “
Purification and characterization of the human g-secretase complex.”
Biochemistry,
2004; 43: 9774-89.
Bihel F, Das C, Bowman MJ, Wolfe MS. “
Discovery of a subnanomolar helical D-tridecapeptide inhibitor of g-secretase.”
J. Med. Chem.,
2004; 47: 3931-3.
Urban S and Wolfe MS. “
Reconstitution of intramembrane proteolysis in vitro reveals pure rhomboid is sufficient for catalysis and specificity.”
Proc. Natl. Acad. Sci. USA,
2005; 102:6, 1883-8.
Kornilova AY, Bihel F, Das C and Wolfe MS. “
The initial substrate binding site of g-secretase is located on presenilin near the active site.”
Proc. Natl. Acad. Sci. USA,
2005; 102:9, 3230-5.
Fraering PC, Ye W, Lavoie MJ, Ostaszewski BL, Selkoe DJ, Wolfe MS. “
g -Secretase substrate selectivity can be modulated directly via interaction with a nucleotide binding site.”
J. Biol. Chem.,
2005; 280(51):41987-96.
Lazarov VK, Fraering PC, Ye W, Wolfe MS, Selkoe DJ, Li H. “
Electron microscopic structure of purified, active gamma-secretase reveals an aqueous intramembrane chamber and two pores.”
Proc. Natl. Acad. Sci. USA,
2006; 103(18):6889-94.
Kornilova AY, Kim J, Laudon H, Wolfe MS. “
Deducing the transmembrane domain organization of presenilin-1 in g-secretase by cysteine disulfide crosslinking.”
Biochemistry,
2006; 45(24):7598-604.
Sato T, Nyborg AC, Iwata N, Diehl TS, Saido TC, Golde TE, Wolfe MS. “
Signal peptide peptidase: biochemical properties and modulation by nonsteroidal anti-inflammatory drugs.”
Biochemistry,
2006; 45(28):8649-56.
Donahue CP, Muratore C, Wu J, Kosik KS, Wolfe MS. “
Stabilization of the tau exon 10 stem loop alters pre-mRNA splicing.”
J. Biol. Chem.,
2007, 281(33): 23303-06.
Narayanan S, Sato T, Wolfe MS. “
A C-terminal region of signal peptide peptidase defines a functional domain for intramembrane aspartyl protease catalysis.”
J. Biol. Chem.,
2007, 282(28): 20172-9.
Selected Reviews and Book Chapters
Esler WP and Wolfe MS. “Portraits
of Alzheimer secretases: new features and familiar faces.” Science 2001, 293,
1449-54.
Wolfe MS. “Therapeutic strategies for Alzheimer’s
disease.”
Nature Reviews Drug Discovery 2002,1, 859-66.
Wolfe MS, Kopan R. “Intramembrane proteolysis: Theme and variations.” Science 2004; 305: 1119-1123
Wolfe MS. “Shutting Down Alzheimer's” Scientific American, May 2006, 72-9
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